Using cotyledon as explants and taking MS as basic medium,different combination of hormones were added to induce adventitious buds in cotyledon culture of Baiyu colorful pepper in vitro.The results showed that the cotyledons by seed propagation were cut to 5 mm2 and cultured on medium of MS + 0.6mg/L NAA.The results of inducing callus were the best.Later,the callus were cut to pieces and cultured on the medium of MS + 0.4mg/L 6BA + 0.2mg/L NAA or the medium of MS + 1.0mg/L KT + 0.2mg/L NAA.The results of inducing adventitious buds were better.