In this experiment,seeds of fine pepper variety were used as test material and they were cultured on MS medium to get aseptic seedlings.Then the cotyledon,hypocotyl and root of aseptic seedlings were adopted as explants and cultured on MS medium with different concentrations of 6-BA and 2,4-D to induce callus.The results showed that the quality and induction rate of callus were better when hypocotyl was used as explants.The optimum medium of callus induction was MS + 0.5mg/L 6-BA + 2.0mg/L 2,4-D.