Aged rice seeds were treated with different time of ultraviolet irradiation and soaked with different concentration of colchicine.The effect of two kinds of factors was analyzed by measuring seed germination rate and amylase activity.Results showed that more than 2 hours ultraviolet irradiation significantly reduced the seed germination rate,but 0.2% and 0.3% concentration colchicine significantly improved the germination rate.Two kinds of different combination treatments significantly promoted the germination rate.In three of the treatment combination,seed germination rate was as high as 92.22%.When seed were treated with colchicine and 2 hours of ultraviolet irradiation at the same time,germination rate did not decline.Amylase activity was significantly improved by different time ultraviolet irradiation.Enzyme activity was significantly improved by 0.2% and 0.4% of colchicine,and 0.3% and 0.5% concentration treatment was significantly higher than those in the control group.Effect of ultraviolet treatment was better than that of colchicine.Among combination treatment,10 groups of enzyme activity increased significantly.Enzyme activity presented two peak value in most of the treatments,which appeared in the second and the fifth day.Enzyme activity reached maximum in the first day or the second day of the treatment.