The fluorescent chromosome banding of Zea mays L.,Glycine max L.,Sesamum indicum L.,Luffa cylindrical Roem,and Arachis hypogaea L.were revealed by DAPI staining.The results showed that there were obvious fluorescent band patterns in maize and peanut,which could facilitate chromosomes recognition.The band patters in sesame and loofah were weaker compared to maize and peanut,which could facilitate some of the chromosomes recognition.There was no clear DAPI band observed in soybean chromosomes.These results showed that there were different DAPI chromosomes banding patterns in different species.