Leaves of ‘Hezuo 918’ tomato were used as explants,and 12 medium of 3 groups were used in this callus inducing test.All the formulas were based on MS medium and different hormones added.The results showed that the effect to induce the callus of ’Hezuo 918’ tomato was the best that using MS + KT 1.5mg/L + NAA 0.2mg/L formula.