The F2 population developed from the cross between two Japonica rice cultivars,’D18S（PTGMS）’ and ’Nian No.2’,was used to identify quantitative trait loci（QTLs） for major agronomic traits including seed setting rate.The results revealed that the segregation of the normal fertile plant and sterile plant subject to the ratio of 3:1,indicating that the trait PTGMS in the population D18S（PTGMS）×Nian No2 is controlled by a independent recessive gene.A total of seven simple sequence repeat（SSR） markers segregated in the F2 population were distributed over the chromosome 7.The PTGMS gene was mapped between the markers RM445 and RM418 with 4.5 cM and 0.7cM interval.Moreover,two QTLs related with plant height and ear length were also mapped to the same position,which contributes to plant height and ear length by 17.5% and 20.0%.In addition,seed setting rate could interpret 83.2% of phenotypic variations.