Na⁺/H⁺ antiporter played a vital role in the process of plant resistant to saline-alkali.In this study,Na⁺/H⁺ antiporter gene NHX1 was cloned and connected to the eukaryotic expression vector pBIGFP,which was transformed into tobacco mediated by Agrobacterium LBA4404.Further molecular detection of experimental results showed that the NHX1 gene was successfully integrated into the genome of tobacco and expressed effectively.