Fragaria×ananassa seedlings in vitro of segments were used as test materials and they were cultured on different propagation medium to study effect of basic medium,hormones,water,the selection of carbohydrate,the amount of agar on the propagation.The results showed that the best medium was MS,in which 2mg/L 6-BA plus 40 g/L white sugar and 5 g/L agar.Tap-water was used in the medium.