Corn seedlings were used as experiment materials to study the effect of different concentration of salicylic acid on some physiological indexes of corn seedlings under chilling stress. The results indicated that the content of MDA and the relative permeability of cell membrane in corn seedling leaves under low temperature reduced by exogenous salicylic acid. At the same time, the content of praline and the SOD activity increased. 0.5 mmol·L-1 salicylic acid was the optimum concentration to corn seedlings under chilling stress. Therefore, salicylic acid could remit the effect of chilling stress on the corn seedlings, and the optimum concentration was 0.5 mmol·L-1.