The rhizosphere soil of raspberry ’Heritage’ of 2, 4, 5 and 7 years old was collected at leaf-expansion, new shoot growth, flower budding, fruit maturity and defoliation stage. The soil enzymes（catalase,urease, phosphatase） activity, soil microbial biomass of carbon and nitrogen and the number of soil microorganisms（bacteria, fungi and actinomycetes） were determined. The results showed that the activities of catalase, urease and phosphatase, microbial biomass of C and N, and the number of soil bacteria presented the trend of increased at first and then decreased. The activities of catalase and urease, soil microbial biomass of C and N, the number of soil bacteria reached the maximum at the flower budding stage, but the phosphatase activity reached the maximum in the fruit maturity period. The number of fungi presented a decrease-increase-decrease trend, and it reached the maximum at fruit maturity period. The dynamic change trend of actinomyces was just the opposite to fungi, increase-decrease-increase, and reached the maximum in flower budding stage.