In this study, a metagenomic library of glyphosate-contaminated soil was constructed, and a glyphosate-resistant clone was obtained from the library with glyphosate resistance screening method. A novel glyphosate oxidoreductase（GOX） gene was found from the positive clone, which was designated as goxA. The length of goxA was 1296 bp, and its encoding product composed of 431 aa. There were six bases difference between goxA and gox. In addition the encoded amino acid sequence of GOXA had two amino acids difference with GOX. Then prokaryotic expression and purification were done. The active stain result showed that GOXA had obvious glyphosate oxidoreductase activity. In short, the goxA identified in this study can become potential candidate genes for developing glyphosate-tolerant transgenic plants.