An efficient regeneration system was established from the explants of leaves of‘Sengara’straw berry（Fragaria ananassa Duch.）. The effects of dark incubation and plant growth regulator combinations on adventitious bud induction were studied. The results showed that the optimum medium fo‘rSongara’leaves induction was MS + 3.0 mg/L 6-BA + 0.2 mg/L 2,4-D, and the induction rate was 73.3%. The average sprouting numbers was up to 2.2. Adventitious bud induction rate was higher when dark cultured for 7 days then the light culture, and the adventitious bud induction rate was 78.2%. For 300 mg/L Cef sterilization,explants differentiation rate was the highest, and Km screening pressure was 20 mg/L for‘Sengala’in adventitious bud differentiation stage.