24 small seed soybean varieties from Jilin Province and Heilongjiang Province were sprouted by sprout machine. The sprout length, hypocotyl length, root length, hypocotyl thickness and soybean sprouts yield were measured and analyzed. The results showed that there existed significant difference among the sprout characteristics of different small seed soybean varieties. Sprout yield was significantly positively correlated with sprout length, hypocotyl length and root length, and was significantly negatively correlated with 100-seed-weight. Two small seed soybean varieties with favorable sprout characteristics were identified,which can be used in soybean sprout production and genetic improvement of sprouted soybean varieties.