盐胁迫对转基因大豆发芽势和发芽率的影响
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S565.104.1

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高产优质抗旱大豆种质资源创新及新品种选育(2009-1)


Effect of Salt Stress on Germinating Energy and Germination Rate of Genetically Modified Soybean
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    摘要:

    本研究以5份转基因大豆株系和2份非转基因大豆株系的种子为试验材料,设置4种盐浓度,采用砂培的方法在实验室进行标准发芽试验,观察不同株系在不同盐浓度条件下萌发和苗期的生长情况,鉴定不同株系耐盐性差异,筛选耐盐株系;取转基因植株幼嫩叶片进行基因组DNA的提取,然后利用PCR技术检测转化的耐盐目的基因是否存在。本研究取得如下研究结果:在相同温度条件下,转基因株系的发芽率均高于未转入目的基因的对照品种,其中BADH-14、BADH-17、CMO-5的耐盐性明显高于对照吉农17。

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    In this study,five transgenic soybean lines and two non-transgenic soybean lines were used as materials to conduct a standard germination examination under four salt concentrations using the method of sand culture. The germination ability and seedling growth were tested and salt tolerance of different transgenic lines was screened. The genomic DNA of transgenic plant lines was extracted from young leaves to detect the target gene by using PCR technology. The results showed that at same temperature conditions,the germination rates of transgenic plant lines under salt condition were higher than that of non-transgenic lines. Lines of ’BADH- 14’,’BADH- 17’ and ’CMO- 5’ show better salt tolerance than their recipient ’Jinong 17’.

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李秀英,王丕武.盐胁迫对转基因大豆发芽势和发芽率的影响[J].东北农业科学,2013,38(6):14-18.

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  • 收稿日期:2013-05-21
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  • 在线发布日期: 2024-12-06
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