To find efficient bio-control bacteria against pathogens of Panax quinquefolium L.,7 strains strongly antagonized pathogens of ginseng were selected from perennial Panax quinquefolium L. rhizosphere soil,all of which had good antagonisms against various pathogens of ginseng and featured broad-spectrum antagonism. The 16SrDNA sequences of the above 7 strains were obtained by amplification and sequencing. Multiple sequence alignment was performed by ClustalX,and the 16SrRNA phylogenetic tree was reconstructed by MEGA5.0. Based on identifications of thallophytic morphological,physiological and biochemical characteristics,strain SJF-5 and SJF-20 were Bacillus methylotrophicus. Strain SJF-8 was Bacillus aerophilus,strain SJF-14 was Bacillus amyloliquefacie,and strain SJF-24 was Bacillus pumilus. Among 2 Brebibacillus strains,strain SJF-6 and SJF-26 both were Brevibacillus borstelensis. This is the first paper reported Bacillus amyloliquefaciens,Brevundimonas sp.,Bacillus methylotrophicus and Bacillus vallismortisis antibiotic against Panax quinquefolium L. pathogeny fungus.