Pot-cultured potato of three cultivars ’Dongnong 311’,’Kexin 13’ and ’Yanshu 4’ was used to study the relationship of drought tolerance and chlorophyll fluorescence parameters during water stress in the shed of drought-resistance. The results indicated that Fo,Fv/Fm,Y（II）,Y（NPQ）,NPQ,ETR value were correlated to relative soil water content. Fm,Y（NO）,qP value were not correlated to relative soil water content. The change of chlorophyll fluorescence parameters was different among three potato cultivars. The drought adaptability of three potato cultivars was ’Dongnong 311’ ＞ ’Kexin 13’ ＞ ’Yanshu 4’ in this study. Chlorophyll fluorescence parameters can be used as index in study of potato’s drought resistance.