The laccase activity of 21 strains belong to basidiomycetes was compared. 3 high laccase-producing level strains were screened out using the plate cultivation and fluid fermentation method,which were Cerrena unicolor,Tyromyces chioneus and Bjerkandera adusta. The laccase activity of the strains cultivated for 15 days were above 300 U/mL. Then the degradation effect of stalk by the 3 strains was studied. The result showed the effect of lignin degradation by B. adusta and T. chioneus were higher,the degradation rate were 66.13% and 61.92%.