Six lines of different colored potato and ‘Zaodabai’ were selected as experimental materials, anthocyanin content and phenylalanine ammonia-lyase（PAL） activity in different organization were determined at 45 d, 60 d, 75 d, 90 d, and 105 d after planting. The correlation of these two indexes was analyzed. The results showed that among different organizations of the same lines or among different clones, where the anthocyanin content was higher, the PAL activity was also higher in entire growing period. The correlation analysis and linear regression models showed that anthocyanin content was positive linear correlated with the activity of PAL. This indicated that PAL is a key enzyme in anthocyanin biosynthesis of color potato.