Seed characteristics of 25 blueberry cultivars were studied in Dalian. The results showed that seed morphology and germinating characteristics were significant different among cultivars. Coefficients of variation of seed length, seed width, seed length /seed width and 1000-grain weight were 9.40%, 8.54%, 7.02% and 9.40%, respectively. Coefficients of variation of onset of seed germination, end of seed germination, mean germination time were 12.38%, 22.22% and 4.90%, respectively. Percentage of germination among varieties varied largely, the coefficient of variation was 52.58%. The percentage of germination of ‘Mead’, ‘Patriot’ and ‘Sunrise’ was higher than other cultivars, which was 77.3%, 73.3% and 60.7%, respectively. The research results can provide a scientific basis for cultivation and breeding of blueberry.