玉米ZmbZIP基因的克隆及表达模式分析
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Q786


Cloning and Expression Pattern Analysis of ZmbZIP in Maize
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    摘要:

    根据玉米碱性亮氨酸拉链(bZIP)基因EST序列设计引物,采用RT-PCR技术从玉米中克隆了一个ZmbZIP基因。研究结果表明:ZmbZIP基因cDNA全长1 300 bp,5'-非编码区长180 bp,3'-非编码区长226 bp,编码区长894 bp,编码297个氨基酸,预测分子量为32.4 KDa,等电点为9.39。氨基酸同源性分析发现,ZmbZIP与谷子的bZIP同源性较高。实时定量PCR检测表明,ZmbZIP基因的表达受脱落酸,干旱和高盐胁迫的诱导,不受低温诱导,说明玉米ZmbZIP基因可能参与玉米对逆境胁迫的应答。

    Abstract:

    According to EST sequence of ZmbZIP, primers were designed. RT-PCR method was used to clone ZmbZIP gene. A gene coding for ZmbZIP was isolated from maize(Zea mays). The full length ZmbZIP cDNA is 1300 bp, including a 180 bp 5’-UTR, an ORF of 894 bp, and a 226 bp 3’-UTR. This cDNA sequence encoded a polypepide of 297 amino acid residues with a predicted molecular mass of 32.4 kDa and a basic isoelectric point of 9.39. The deduced amino acid sequence had a high homology with bZIP from Setaria italica. Quantitative real-time PCR results showed that the ZmbZIP gene expressed was induced by abscisic acid, high salinity, and drought treatment, not induced by low temperature. The expression patterns of ZmbZIP under different stresses suggested that this gene might be involved in the regulations of maize to stresses.

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李横江.玉米ZmbZIP基因的克隆及表达模式分析[J].东北农业科学,2014,39(2):25-27,46.

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  • 收稿日期:2013-08-30
  • 在线发布日期: 2024-12-06
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