Abstract:Secondary metabolites from DW22, an endophytic fungus isolated from root-zone soil collected in Northwest of Liaoning, were extracted with 20%, 40%, 60%, 80% and 100% ethanol, respectively. The ethanol extracts were numbered with 22-1, 22-2, 22-3, 22-4 and 22-5 in order. Inhibitory activities to Magnaporthe grisea were detected, and the variances and significant differences were analyzed. The results showed that 20% ethanol extract of secondary metabolites from DW22 endophytic fungus had better inhibiting effect to Magnaporthe grisea.