番茄叶片高频再生体系的建立
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S641.2

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吉林省农业科技创新工程优秀团队项目(C2228010205)


Establishment of Leaf-Based High-Frequency Regeneration Procedure for Tomato
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    摘要:

    以番茄叶片为外植体,比较不同基因型在含有不同激素浓度配比的培养基上的再生能力,目的在于获得某特定番茄品种的高频再生体系,并结合该番茄品种对除草剂的敏感性实验,为建立番茄遗传转化受体系统奠定基础。结果表明,‘绿樱桃’在含有3.0 mg/L 6-苄基氨基嘌呤(6-benzyl amino purine,6-BA)和0.2 mg/L 3-吲哚乙酸(indole-3-acetic acid,IAA)的MS培养基中再生最好,愈伤组织形成率为96.88%,不定芽诱导率达90.63%;每个外植体的平均不定芽诱导率为252%,其最适的生根培养基为MS附加IAA 0.2mg/L;除草剂草丁膦的致死浓度为1.5 mg/L。

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    Tomato leaves were used for explants and regeneration capacity of different genotypes on medium with various hormone combinations was investigated in this study which aimed at developing a high-frequency regeneration system for a certain tomato cultivar. In addition, susceptibility of used specific genotype to herbicide was examined to provide valuable information for establishment of tomato transformation. The results showed that the cultivar ’Green cherry’ had the highest regeneration capacity on MS medium supplemented with 3.0 mg/L 6-BA and 0.2 mg/L IAA. In these conditions callus and shoot induction rates were 96.88% and 90.63%,respectively. The shoot induction rate per explant averaged 252% after 50 days of growth. Furthermore MS medium supplemented with 0.2 mg/L IAA was found to be optimal for rooting of ’Green cherry’. Finally, it was found that phosphinothricin was lethal to ’Green cherry’ at concentrations of 1.5 mg/L.

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张玉英,韦正乙,王云鹏,林春晶,邢少辰.番茄叶片高频再生体系的建立[J].东北农业科学,2014,39(2):78-82,86.

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  • 收稿日期:2013-10-31
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  • 在线发布日期: 2024-12-06
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