Tomato leaves were used for explants and regeneration capacity of different genotypes on medium with various hormone combinations was investigated in this study which aimed at developing a high-frequency regeneration system for a certain tomato cultivar. In addition, susceptibility of used specific genotype to herbicide was examined to provide valuable information for establishment of tomato transformation. The results showed that the cultivar ’Green cherry’ had the highest regeneration capacity on MS medium supplemented with 3.0 mg/L 6-BA and 0.2 mg/L IAA. In these conditions callus and shoot induction rates were 96.88% and 90.63%,respectively. The shoot induction rate per explant averaged 252% after 50 days of growth. Furthermore MS medium supplemented with 0.2 mg/L IAA was found to be optimal for rooting of ’Green cherry’. Finally, it was found that phosphinothricin was lethal to ’Green cherry’ at concentrations of 1.5 mg/L.