Using eight maize lines as target genotypes, a highly efficient regeneration system was developed with MS being used as basal culture medium. Effects of genotypes, immature embryos size and hormone concentrations on the induction of embryogenic callus were investigated in this work. The results showed that all experimental maize materials could be induced to form callus. However, the rates of callus induction, embryogenic callus induction and retention were different among different genotypes. Under our experimental conditions, the best induction and subculture of embryogenic callus was obtained form ‘Denghai No.11’ maize hybrid. Furthermore, some important factors were optimized. The results showed that the immature embryos of 1.0-2.0 mm in length in DH11 were optimal explants. Addition of 2-3 mg/L 2,4-D in induction medium significantly stimulated embryogenic callus of DH11. And the differentiation medium supplemented with 0.5mg/L 6-BA had very great promoting effect on inducing differentiation.