In order to develop scientific and rational fertilizer application in farmland, improve nutrient utilization, promote the development of variable fertilization technology, taking the soil nutrients of the cultivated land in Gaojia Village and Jinshan Village of Lishu Country of Jilin Province as an example, the effects of conservation tillage patterns on spatial variability of soil nutrients in black soil was studied in the paper using geostatistics models and GIS technology. The results showed that the spatial variability of soil nutrients under the conservation tillage was greater than that under the conventional tillage, and its structure was more stable than that under the traditional mode, so it has very strong spatial autocorrelation. Except Olsen-P, the biggest range of the soil nutrients under the conventional tillage mode was less than the one under the conservation tillage mode in all. The picture of spatial variability of soil nutrients manifested that soil nutrients distributed as the agglomerate under the conservation tillage mode, but it distributed as ribbon which was perpendicular to ridge under the conventional tillage mode. The spatial variability of soil nutrients under the conservation tillage was more easily influenced by the natural factors, the spatial variability of soil nutrients under the conventional tillage was more greatly influenced by artificially random factors. The spatial variability of soil nutrients under conservation tillage was greater than that under the conventional tillage, which structure and spatial autocorrelation were very strong, indicating that precision fertilization was more applied to the conservation tillage.