Field trial was adopted to study maize yield and nitrogen transportation among organs under different nitrogen fertilizer application. This study aimed at making clear effect of nitrogen on maize yield and characteristics of nitrogen uptake and transportation to supply basis for realizing proper application of nitrogen.The results showed that proper application of nitrogen can raise maize yield significantly, add dry matter accumulation, and boost nitrogen content in each organ. Nitrogen use efficiency in common application dose treatment was much higher than that of high application dose treatment.