A preliminary study on the antibacterial activity and antibacterial mechanism for the antagonistic effect on Fusarium oxysporum wilt bio-control agent XM-10 was tested on 9 pathogens,such as Fusarium graminearum,Physalospora piricola,Sclerotinia sclerotiorum,etc.It was identified according to the morphology,cultural characters,physiological and biochemical characteristics and 16 S rRNA sequence analysis.The results showed that the inhibitory effect of XM-10 strains to 9 species of pathogenic bacteria Was between 47.8%and 87.6%,in Which the inhaibitory effect to Fusarium graminearum,Fusarium oxysporum were 87.6 and 83.7,respectively.The antibacterial belt was between 4.9 mm and 6.9mm It was identified as yellow grey streptomycete according to morphology and 16 S rRNA sequence analysis.It was concluded that bio-control strain XM-10 was streptomycete which had wide antibacterial effect.It was of great potential in bio-control of many diseases.