In order to offer donor cells for porcine transgenic cloning by nuclear transferring,fibroblast cells were isolated by attaching tissue explants from porcine fetal.The plasmid containing green-fluorescent protein cDNA was transfected into porcine fetal fibroblast by Lipofectmine.Cell clones were obtained after screening by G418.The recombinant of extrogenous DNA was identified by polymerase chain reaction,the extrogenous DNA expression was identified by fluorescence microscope.Results showed that primary porcine fibroblast cells were isolated from the tissue cultured for 9 days,and the transgenic cells were obtained after G418 selection for 9 days.Identification of the transgene in the cell clones was examined by PCR and the exogenous DNA had been integrated into genome.The transgenic cell line could express green-fluorescent protein.These results have paved the way to obtain the newtransgenic porcine by nuclear transfer in the future.