Reintroducing leguminous forage resulted in effective restoration of degraded grassland and improvement of nutritional composition of grassland community.The effects of two rhizobia applications and three seeding rates(0.75 g·m^-2,1.12g·m^-2,1.50g·m^-2on reseeding Medicago sativa Zhongmu no.1 wereinvestigated in this study.The results indicated rhizobia application promoted seedling emergence percentage and survivorship,and improved the seedling height,tillers,leaf area,dry weight and active rhizobia per seedling significantly.However,seeding rate only affected seedling height and dry weight of per square meters significantly,and the largest seedling survivorship and dry weight of per seedling occurred in middle seeding rate(1.12g·m^-2.Our study suggested that 1.12 g·m^-2 was the suitable reseeding rate of Zhongmu no.1 to degraded grassland.In order to obtain successful seedling establishment of reseeding Medic agosativa to the grassland restoration,it is necessary to use the local adaptive rhizobia and choose the optimumseeding rate.