In order to study the effect of Trichoderma solution on the plant growth, soybeans, cucumbers and lettuce seeds were soaked with Trichoderma solution. The results showed the appropriate concentration of Trichoderma solution for seeds of soybean, cucumber, lettuce were 80 mg/L, 60 mg/L and 40 mg/L, respectively. After soaked in 80 mg/L Trichoderma solution, soybean seeds germination index was 1.83 times, the root length was 1.22 times and vigor index was 2.02 times of the control. After soaked in 60 mg/L Trichoderma solution, germination index of cucumber seed were 4.90 times, the root length was 2.34 times and vigor index was 9.75 times of the control. After soaked in 40 mg/L Trichoderma solution, germination index of lettuce was 1.35 times, the root length was 1.84 times and vigor index was 1.55 times of the control. Whereas seed soaking with 80~100 mg/L Trichoderma solution has obvious inhibitory effect to lettuce seed germination.