Two Bacillus thuringiensis Cry1C^* and Cry2A^* genes（Bt） on individual plasmids were simultaneously introduced into Japonica rice variety ‘Jijing 88’ by Agrobacterium-mediated co-transformation. Five hundred and ninety eight independent basta-resistance transgenic plants were obtained by 30 mg/L basta-screening.PCR verification revealed that 35 were co-transgenic plants harboring the two Bt genes, and the co-transformation frequency was 6.28%. Three of two-Bt gene single copy insertion lines were identified among 35 co-transgenic lines by Southern blot analysis. RT-PCR analysis demonstrated that the two Bt transgenes were simultaneously expressed in these 3 lines at the transcription level.