Green house cultivation method was conducted to determine effect of the compound preparation of bio-pesticide and chemical fertilizer on rice seedling quality in the paper. The results showed that soil pH was 4.5 to 5.5 during rice seedling growth period. Bound water content was higher, width of stem bottom, shoot and root fresh and dry weight per hundred plants and chlorophyll content were not different compared with chemical preparation sold on the market. Strong seedling can be cultivated by using this compound preparation.