To provide more resources for the biological control of grape downy mildew, the antagonistic fungi against the disease were screened in this study. By exploiting streak plate method, 87 fungous strains from the health grape leaves were separated. After primary screening by a dual culture technique, 4 strains exhibiting antagonistic activities against pepper phytophthora blight were screened. By method of the leaf disc for secondary screening, Y29 was screened. Y29 had the better inhibitory effect, which the bio-control efficacy was 72.9%. Based on its colony morphology, mycelial morphology, sporogenous structure, D1/D2 region of 26 S rDNA sequence and phylogenetic systematics, the Y29 strain was identified as Leptosphaerulina australis.