Using eight species of peanut germplasm resources as the experimental materials in this experiment,the appropriate DNA extraction method was explored. 4 sets of reaction system were selected through reading a largeamount of papers and preliminary screening. Different primers were screened in the reaction system. SSR-PCR sys-tem suitable to peanut was finally established, in which for 10 μL, 25 ng DNA, 1buffer, 0.15μmol/L primer, 200μmol/L d NTP, and 0.5UTaq. A PCR system was finally established, in which pre-denaturing 5 min at 94 ℃, denaturing for 1 min at 94℃, annealing for 30 s at 55℃, extending for 1 min at 72℃, 35 cycles, extending for 5 min at 72℃.