齐齐哈尔玉米穗腐病病原菌的鉴定和致病性测定
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S435.131.4+9

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吉林省科技厅项目(c42030803)


Identification of Pathogen and Pathogenicity of Maize Ear Rot in Qiqihaer City
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    摘要:

    为明确齐齐哈尔市玉米穗腐病病原菌,于2013年自黑龙江省齐齐哈尔市玉米种植区采集玉米穗腐病样品,进行病原物分离、鉴定及致病性测定研究。结果表明:分离的菌株菌落形态、分生孢子形态与禾谷镰孢菌相同,但不分泌色素;ITS基因序列与Genebank登记中的禾谷镰孢菌HQ671191.1同源性为100%;系统发育树状图结果显示该菌株与禾谷镰孢菌位于系统发育树的同一分支;按照科赫氏法则进行致病性测定,证实分离的病原菌可造成严重的玉米果穗腐烂。因此将该菌株命名为禾谷镰孢菌齐齐哈尔变种。

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    To identify pathogens of maize ear rot in Qiqihaer city, samples were collected from maize fields in Qiqihaer City in December, 2013. These pathogens were isolated and identified, and their pathogenicity tested. The results showed that colony morphology and conidial morphology of the isolated pathogen Q-1 was similar with Fusarium graminearum, but it did not secrete pigment. The ITS sequence max similarity was 100% with Fusarium graminearum(Genebank No: HQ671191.1). The phylogenetic tree showed that the Q-1 was in the same cluster as F. graminearum. The pathogenicity test of Q-1 indicated that Q-1 was verified to maize ear according to Koch’s Pos-tulot. So the Q-1 was named as Fusarium graminearum Qiqihaer.

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刘振库,贾娇,苏前富,孟玲敏,晋齐鸣.齐齐哈尔玉米穗腐病病原菌的鉴定和致病性测定[J].东北农业科学,2014,39(6):28-30.

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  • 收稿日期:2014-06-02
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  • 在线发布日期: 2024-12-06
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