虎纹蛙舌肥大细胞组化及免疫组化
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Q952

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福建省教育厅项目(JA11092)


Studies on the Mast Cells in the Tongue of Frog (Rana tigrina rugulosa) by Histochemical and Immunohistochemical Method
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    摘要:

    应用改良甲苯胺蓝(MTB)、阿尔新蓝-藏红O(AB/SO)、甲基绿-派洛宁(MG-P)、天青Ⅱ-伊红-瑞氏混合液等4种组化染色技术进行染色,并用小鼠抗人肥大细胞类胰蛋白酶单克隆抗体AAl通过ElivisionTM免疫组化染色方法,对虎纹蛙舌头肥大细胞进行染色。结果表明:虎纹蛙舌头中的肥大细胞,大小形态不一。虎纹蛙舌组织中分布有大量肥大细胞,主要分布于固有层的结缔组织中和舌头腺体之间,肌层也可见到大量的肥大细胞,并且有沿血管分布的特点。在用ElivisionTM免疫组化染色方法中,单克隆抗体AAl与虎纹蛙舌头组织中肥大细胞中的类胰蛋白酶有良好的交叉反应。类胰蛋白酶阳性细胞数量很少,分布于舌组织固有层和腺体之间。而人胃癌间质中可见多量类胰蛋白酶阳性细胞。

    Abstract:

    The mast cells of the back, abdominal skin and tongue of the frog, Rana tigrina rugulosa, werestained using modified toluidine blue(MTB), Alcian blue Safranin O(AB/SO), methyl green- Pyronine(MG- P),Azure Ⅱ- Eosin-Wright mixture and a murine monoclonal antibody(AA1) that raised against human mast celltryptase by Elivision TM immunohistochemical. The results showed that the mast cells in the tongue of the frog haddifferent size and form. There were lots of mast cells in the tongue tissue of the frog which were mainly distributedbetween the connective tissue of lamina propria and lingual gland. Lots distributed in muscular layer and intendedto be around blood vessels. With Elivision TM immunohistochemical staining method, the monoclonal antibody AAland the tryptase of the mast cells in the tongue tissue of the frog had an excellent cross-reaction. Small amount ofthe positive tryptase cells were found in the frog and mostly distributed between the lamina propria and gland of thetongue tissue. There were lots of tryptase-positive mast cell in the mesenchyme of the human gastric cancer.

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林旋,王寿昆,林树根,陈梅芳,李玲玲.虎纹蛙舌肥大细胞组化及免疫组化[J].东北农业科学,2014,39(6):81-84.

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  • 收稿日期:2014-05-08
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  • 在线发布日期: 2024-12-06
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