The mast cells of the back, abdominal skin and tongue of the frog, Rana tigrina rugulosa, werestained using modified toluidine blue（MTB）, Alcian blue Safranin O（AB/SO）, methyl green- Pyronine（MG- P）,Azure Ⅱ- Eosin-Wright mixture and a murine monoclonal antibody（AA1） that raised against human mast celltryptase by Elivision TM immunohistochemical. The results showed that the mast cells in the tongue of the frog haddifferent size and form. There were lots of mast cells in the tongue tissue of the frog which were mainly distributedbetween the connective tissue of lamina propria and lingual gland. Lots distributed in muscular layer and intendedto be around blood vessels. With Elivision TM immunohistochemical staining method, the monoclonal antibody AAland the tryptase of the mast cells in the tongue tissue of the frog had an excellent cross-reaction. Small amount ofthe positive tryptase cells were found in the frog and mostly distributed between the lamina propria and gland of thetongue tissue. There were lots of tryptase-positive mast cell in the mesenchyme of the human gastric cancer.