Using Xianyu 335 as material, effect of maize tiller on yield was studied in cold and humid region ofeastern Jilin Province. The variance analysis results showed that the yield between removed and retained maize til-ler was not significant different under the plants density of 52 400~59 500 plants/hm². So, retained maize tillerdidn’t affect the yield of maize, moreover it was benefit to enhancing the producing efficiency of Maize in this region.