[Objective] In order to study the bio-control bacteria screened from forest soil in Dahei Mountain.Bacteria were identified by morphological, 16 S rDNA, and its antimicrobial protein was separated. [Method] Theplate-confrontation method and antibacterial circle method were used for preliminary screening and antimicrobialtesting in vitro, respectively. The taxonomic identification of strain K1 was studied by analyzing its morphology char-acteristics, physiological and biochemical characteristics, and 16 S rDNA gene sequence. Protein was separated bySephacryI S-200. [Results] K1 had a stable inhibitory effect against Botrytis cinerea. Inhibition zone diameter was 45 mm. From the taxonomic identification result, this bacterium was identified as Bacillus amyloliquefaciens. An anti-microbial protein was isolated by SephacryI S-200. And SDS-PAGE results showed that molecular weight of proteinwas about 55 kDa. [Conclusion] A bio-control Bacillus amyloliquefaciens K1 was screened. An antibacterial proteinwith 55 kDa was isolated.