Cytotoxic CctA gene was amplified by PCR according to the GenBank Clostridium chauvoei cytotoxic CctA gene reference sequence. After cloning and sequencing, the cytotoxic CctA protein physical and chemical prop-erties, the structure and function domain, protein secondary structure and tertiary structure and antigen epitope andother important parameters were predicted and analyzed by bioinformatics methods. The result showed that com-pared to the reference sequence（JQ692583） there were three point mutations, the sequence homologies of nucleo-tides and amino acids were both 99.4%. The theoretical isoelectric point of protein was 8.00. Cytotoxic CctA proteindidn’t exist in the transmembrane region and signal peptide sequence. The secondary structures were mainly in be-ta-folding and random curl, and there were 4 main antigenic epitope regions. This study provided a reference for fur-ther research on cytotoxic CctA protein function, and laid the groundwork for Clostridium chauvoei monoclonal anti-bodies, synthetic peptide vaccine research and development.