In order to rise up the gas production in process of anaerobic fermentation of agricultural waste, a setof microbial inoculum（CPB） which can degrade maize straw was build up and inoculated into the fermentation sys-tem filled with cow dung and straws to investigate the effects in gas production. Different amounts of microbial inocu-lum 0（T₁）,100 mL（T₂）, 200 mL（T₃） and 300 mL（T₄） were inoculated and the gas production was measured. The gasproduction was increased by 6.6%, 19.6% and 16.6% compared to no-CPB inoculated control, respectively. Degra-dation of cellulose-riched materials may be correlated with the lytic ability of microbial inoculum. As well as, DG-GE results shows that 6 strains were indentified and incubated except 1 strain which can not be incubated.