Fresh and dried pollen of seeded and seedless grape cultivars were used as material in the experi-ment and they were stored at 25℃, 5℃, 0℃,-20℃ and-40℃. Using the same solid medium of sucrose 200 g/L +boric acid 50 mg/L + agar 8g/L as nutrient medium, pollen germination in vitro culture was conducted. The resultsshowed that there were obvious differences in pollen viability between different cultivars at the beginning of storage,and pollen viability of seeded grape cultivars was higher than that of seedless grape cultivars. As storage time ex-tended, pollen viability of different temperature storage treatments decreased, and the pollen viability of-20℃ and-40℃treatments decreased slowly, while pollen viability stored at 25℃ decreased rapidly.