TS201.3
现代农业产业技术体系专项资金资助(CARS-37);国家自然科学基金青年科研基金(31201394);吉林省农业科学院重大项目(C42070302);吉林省农业科技创新工程项目(C42070303)
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内蒙古奶豆腐中分离的一株优良益生特性的乳杆菌菌株CH4经API 50 CHL和16S rDNA序列分析鉴定为发酵乳杆菌(Lactobacillus fermentum)。本研究分析了益生元对CH4生长的促进作用。单因子试验表明,棉籽糖对CH4的促生长能力最强,添加量为1.5%,培养时间为10 h有利于菌株CH4的生长。利用正交试验进行验证,最终确定发酵乳杆菌CH4的生长条件为:培养基中添加1.5%棉籽糖,培养11 h,发酵乳杆菌CH4的活菌数达到8.65×1010CFU/mL,与未添加低聚糖培养时相比提高40倍。
Potential probiotic characterization of Lactobacillus strains CH4 isolated from Inner Mongolia Hu-rood cheese was identified as L. fermentum by API 50 CHL and 16 S rDNA sequence analysis. In this study, the ef-fects of prebiotics types, added volume, and incubation time for this strain were explored by ingle factor and orthogo-nal experiments. Results showed viable count of L. fermentum CH4 was 8.65×1010CFU/mL in medium with 1.5%raffinose addition and 11 h incubation and increased 40-fold compared to culturing without oligosaccharides.
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牛春华,赵玉娟,张健,马爽,李盛钰.益生元低聚糖对发酵乳杆菌CH4的促生长作用[J].东北农业科学,2015,40(2):105-109.
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- 收稿日期:2014-10-15
- 在线发布日期: 2024-12-06
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