Adopting random block design, effect of unbalanced nitrogen fertilizer application on yield and grainfilling rate of maize was studied. The results showed that compared with normal nitrogen fertilizer application, drymatter accumulation of the treatment which was no nitrogen fertilizer application was greatly affected. 15 days aftersilking stage, dry matter accumulation of the no nitrogen fertilizer application treatment decreased by 37.8% of thecontrol. Later, the difference of both enlarged. Except for rows per ear, the difference of other factors of yield andyield of no nitrogen fertilizer application compared with normal nitrogen fertilizer application was very significantlevel. Yield decreased 68.3%, N accumulation of grain decreased 76.5%. The maximum grain filling and mean grainfilling rate decreased. The time of maximum grain filling appeared earlier. Active grain filling period shortened ap-proximately 13 days. Compared with normal nitrogen fertilizer application, dry matter accumulation of the high nitro-gen treatment was affected a little, which increased a little. Yield increased 4.2%, N accumulation of grain in-creased 19.5%. The maximum grain filling and mean grain filling rate increased a little. The time of maximum grainfilling appeared earlier. Active grain filling period shortened.