Effect of different fertilization patterns on organic matter, available N, P and K contents of upper andsub-upper soil was studied on Gongzhuling Long-Term Position Experimental Station of Jilin Province. The resultsshowed that soil organic matter, available N, P and K contents increased in organic and inorganic fertilizers mixedtreatment, and soil organic matter, available P and K increased significantly in incremental organic and inorganicfertilizer treatment. Soil available N content was the highest in the common organic and inorganic fertilizers andcrop rotation patterns treatment. The nutrient contents remained the same level before the test in leisure soil treat-ment. The soil organic matter, available N, P and K contents did not change obviously in the straw with inorganic fer-tilizer NPK treatment. In short, rational fertilization will maintain soil properties and production capacity in longterm, and this is favorable to sustainable agricultural development, especially applying a combination of organic andinorganic fertilizers.