Using Gisela as experimental materials, the effect of IBA on rooting of in vitro cherry were re-searched. The results showed that:（1） IBA had significant influence on static index of average length, differentiationratio and rooting coefficient and dynamic index of differentiation speed and regeneration speed of Gisela（P＜0.01）;（2） The root Indexes increased firstly and decreased subsequently with the concentration of IBA increased and thetrend of quadratic regression equations was similar. Through regression analysis, rooting in vitro ‘Gesila 5’and ‘Gesila 6’was better when the concentration of IBA was 1.3791~1.4231 mg/L and 1.2931~1.4239 mg/L, respec-tively.