霜霉病菌诱导PRs基因在大白菜中的表达
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S634.1

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山东省自然科学基金项目(ZR2012cq019)


PRs Genes Expression in Chinese cabbage Induction by Peronospora parasitica
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    摘要:

    为研究病程相关蛋白(PRs)基因与植物对霜霉病原菌抗性关系,本研究以大白菜抗病品种为材料,采用实时定量PCR技术检测接种Peronospora parasitica后4种PRs基因的表达。结果表明,P.parasitica接种处理显著诱导了PR-4和class Ⅳ几丁质酶基因在大白菜抗病品种中的表达,β-1,3-葡聚糖基因的表达却呈现双峰曲线特点,PR-1基因表达量没有变化。PRs基因的表达特点表明其在大白菜对霜霉病害抗性中起到了不同的作用。

    Abstract:

    In order to investigate the relationship between pathogeneses-related proteins(PRs) genes and plant resistance against Peronospora parasitica, four PRs genes expression were examined after inoculation with Peronospora parasitica in resistant Chinese cabbage variety by using real-time quantitative PCR(RT-PCR) technology. Results showed that PR-4 gene and class Ⅳ chitinase genes expression were significantly induced by inoculation treat-ment, β-1, 3-glucanase gene expression showed two single peaks, but PR-1 gene expression showed no change by inoculation treatment. PRs genes transcription in resistant variety showed they may play different roles in Chinese cabbage resistance against Peronospora parasitica.

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陈晓峰,马清华,牟晋华,王百川.霜霉病菌诱导PRs基因在大白菜中的表达[J].东北农业科学,2015,40(4):60-64.

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  • 收稿日期:2014-12-28
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  • 在线发布日期: 2024-12-06
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