Sika deer Insulin Growth Factor（IGF-1） mature peptide gene was expressed and purified by prokary-otic expression vector p ET-30 a in this study. Recombinant IGF-1 mature peptide protein from sika deer was ob-tained after splitting decomposition of hydroxylamine and protein renaturation. Chicken embryo fibroblast cell and chicken embryo kidney cell proliferation was determined by MTT assay after recombinant IGF-1 mature pep-tide treatment. The results showed that recombinant IGF-1 mature peptide promoted the proliferation of CEF cell indifferent concentrations. Among them, the most significant impact on CEF cell proliferation was at 48 h, and the finalconcentration was 200 ng/mL. But the effect of recombinant IGF-1 on CEK cell was not significant in different con-centrations and different treatment time. Our findings indicated that IGF-1 mature peptide after renaturation promoted chicken embryo fibroblast proliferation significantly, but its influence on chicken embryo kidney cell proliferation was not significant.