Nitrogen plays an important role in the growth and development of cucumber. In order to study the distribution of nitrogen during the cucumber growth in greenhouse, in the intelligent greenhouse, a test was conduct-ed with bagged perlite, fertilized by Hoagland nutrient solution, T1:1∶200;T2:1∶100;T3:1∶50;T4:1∶30. Throughdestructive test, content of nitrogen in organs was determined during cucumber growth. According to the experi-ments of summer and autumn, nitrogen distribution during cucumber growth and development was improved by T3 treatment. The study’s conclusions provide the theoretical support to N fertilizer application during cucumber growth in greenhouse.