This research is aim to prolong the storage time of powder formulation of Beauveria bassiana. The im-pact factors include the temperature and the reagent. The germination rate, the virulence to Ostrinia furnacalis. andthe activity of Pr1 of conidia in the Beauveria bassiana powder formulation were detected. The results showed thatthe best Beauveria bassiana powder formulation was number 5: the 95% higher concentration spores powder of Beauveria bassiana. plus 2% maltose and 3% peptone. The optimum storage temperature was-20℃. The maltose andpeptone is the important carbon and nitrogen source to Beauveria bassiana, which have the protective function to pre-serve the spore activity of Beauveria bassiana.