Germination and growth of tomato gray leaf spot pathogen and control effect of common chemical fungicides was determined in different temperature and light by growth rate method. Biocontrol strains were screenedby confrontation test. The results showed that the optimal growth temperature of tomato gray leaf spot was 30℃ andthe light did not significantly affect its growth. Fungicide A was best and the inhibition rate reached 100% at 20℃and 25℃. The pathogen germination needed 4d with A1 concentration and 3d with A2 concentration at 30℃.The inhibition rate of Rhizopus stolonifer, Rhizopus nigricans and Trichoderma pseudokoningii against tomato gray leaf spotpathogen was more than 80% and Rhizopus stolonifer reached 87.68%. Pathogen colony atrophied and mycelium digested at the junction of pathogen when dealing with Trichoderma pseudokoningii.