The differential expression gene and gene expression pattern of the rice cultivar with different salineand alkaline treatments were studied by sequencing analysis of ‘Teyou 12’. At the same time, the functional classi-fication and annotation of the differentially expressed genes were classified by biological process. The resultsshowed that the differential expression gene was mainly involved in the oxidation reduction, response to osmoticstress, response to stress, response to oxidative stress, secondary metabolic process, response to stimulus, responseto salt stress, response to chemical stimulus, and response to abiotic stimulus, etc.